Product Overview
Universal U+ Probe qPCR Master Mix (ONE TUBE) is a fully premixed, probe-based qPCR reagent designed for convenient and reliable DNA amplification and detection. The optimized formulation integrates key reaction components into a single tube and supports the pre-addition of primers and probes, helping simplify workflow and reduce manual pipetting steps.
Developed through systematic optimization of molecular enzymes, reaction chemistry, and manufacturing processes, this master mix delivers stable performance, broad assay compatibility, and support for rapid qPCR protocols. It is suitable for the development and production of molecular diagnostic assays, research applications, and routine nucleic acid testing workflows.
Key Features
Long-Term Stability
Optimized fully premixed formulation maintains stable performance after storage at 37°C for up to 28 days, supporting reagent robustness during transportation and storage.
Broad Compatibility
Compatible with a wide range of DNA targets and probe-based qPCR assays, making it suitable for diverse detection applications.
Ultra-Fast qPCR
Supports rapid qPCR protocols with detection completed in as little as 30 minutes, helping improve laboratory throughput.
High Sensitivity
Enables reliable detection of low-copy DNA templates, supporting sensitive target identification.
Easy-to-Use Workflow
Allows primers and probes to be pre-mixed into the reaction system, reducing preparation steps and improving operational convenience.
Applications
• Probe-based quantitative PCR (qPCR) assays
• Multiplex DNA target detection
• Molecular diagnostic assay development and manufacturing
• Pathogen nucleic acid detection
• Research laboratories and testing facilities
• Routine DNA analysis workflows requiring simplified reagent preparation
Performance Data
Long-Term Stability
The fully premixed qPCR system maintains stable amplification performance after 28 days of storage at 37°C, demonstrating strong formulation stability under accelerated storage conditions.
Broad Compatibility
Validated with multiple DNA targets, showing consistent amplification performance across different assay systems.
Ultra-Fast Detection
Supports rapid protocol detection
High Sensitivity
Demonstrates reliable detection of low-copy DNA templates, including single-digit copy levels under validated assay conditions.
FAQ
Q1: What types of samples are compatible with HMD3808?
A1: HMD3808 is a resistant DNA MIX suitable for direct amplification applications. It supports challenging samples such as swabs, blood and sputum samples.
Q2: What makes HMD3808 suitable for direct PCR detection?
A2: HMD3808 features strong inhibitor tolerance and stable amplification performance, enabling reliable PCR detection in complex sample backgrounds.
Q3: Which resistant RNA MIX and DNA MIX are available, and what types of samples can they tolerate?
A3: The recommended resistant RNA MIX is HMD6906, which supports direct amplification of swab and stool samples, as well as extracted samples from swabs, blood and diarrhea specimens.
The recommended resistant DNA MIX includes HMD3808, HMD6801 and HMD3819, which support direct amplification of samples such as swabs, blood and sputum.
